Coding
Part:BBa_K1982009:Design
Designed by: Zexu Li Group: iGEM16_NEU-China (2016-10-09)
Eukaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 405
Illegal BglII site found at 864
Illegal BamHI site found at 1343 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 289
Illegal AgeI site found at 1018 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 641
Illegal BsaI.rc site found at 50
Illegal SapI.rc site found at 158
Design Notes
mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTg
Source
The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.